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revertra ce reverse transcription enzyme  (Toyobo)


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    Structured Review

    Toyobo revertra ce reverse transcription enzyme
    Revertra Ce Reverse Transcription Enzyme, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 9063 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/revertra+ce+reverse+transcription+enzyme/ReverTra+Ace+qPCR+RT+Kit/pm25150399-42-61-66
    Average 99 stars, based on 9063 article reviews
    revertra ce reverse transcription enzyme - by Bioz Stars, 2026-09
    99/100 stars

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    Extraction:

    Article Title: Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish.
    Article Snippet: .. For mRNA extraction, the fish were euthanized by an overdose 0.1%) of MS-222 for 10 min, and spinal cords within 5 mm around he lesion site were collected from at least 3 fish per time point. otal RNA was extracted using Sepasol-RNA I Super G (Nacalai esque, Kyoto, Japan), according to the manufacturer’s instructions. ingle-stranded cDNAs were then synthesized using the ReverTra ce reverse transcription enzyme (Toyobo, Osaka, Japan). .. RT-PCR f the sox2 mRNA in zebrafish spinal cords was conducted using ebrafish sox2-specific primers (forward, 5′-CGC TCC AGT ACA ACT CA TGA CC-3′; reverse, 5′-TTA CAT ATG CGA TAA GGG AAT CGT GC′) and SapphireAmp Fast PCR Master Mix (Takara, Shiga, Japan). he resultant sox2 PCR products were electrophoresed on agarose els, visualized with ethidium bromide, and captured using the eneGenius 2 Bio Imaging System (Syngene, MD, USA).

    Synthesized:

    Article Title: Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish.
    Article Snippet: .. For mRNA extraction, the fish were euthanized by an overdose 0.1%) of MS-222 for 10 min, and spinal cords within 5 mm around he lesion site were collected from at least 3 fish per time point. otal RNA was extracted using Sepasol-RNA I Super G (Nacalai esque, Kyoto, Japan), according to the manufacturer’s instructions. ingle-stranded cDNAs were then synthesized using the ReverTra ce reverse transcription enzyme (Toyobo, Osaka, Japan). .. RT-PCR f the sox2 mRNA in zebrafish spinal cords was conducted using ebrafish sox2-specific primers (forward, 5′-CGC TCC AGT ACA ACT CA TGA CC-3′; reverse, 5′-TTA CAT ATG CGA TAA GGG AAT CGT GC′) and SapphireAmp Fast PCR Master Mix (Takara, Shiga, Japan). he resultant sox2 PCR products were electrophoresed on agarose els, visualized with ethidium bromide, and captured using the eneGenius 2 Bio Imaging System (Syngene, MD, USA).

    Reverse Transcription:

    Article Title: Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish.
    Article Snippet: .. For mRNA extraction, the fish were euthanized by an overdose 0.1%) of MS-222 for 10 min, and spinal cords within 5 mm around he lesion site were collected from at least 3 fish per time point. otal RNA was extracted using Sepasol-RNA I Super G (Nacalai esque, Kyoto, Japan), according to the manufacturer’s instructions. ingle-stranded cDNAs were then synthesized using the ReverTra ce reverse transcription enzyme (Toyobo, Osaka, Japan). .. RT-PCR f the sox2 mRNA in zebrafish spinal cords was conducted using ebrafish sox2-specific primers (forward, 5′-CGC TCC AGT ACA ACT CA TGA CC-3′; reverse, 5′-TTA CAT ATG CGA TAA GGG AAT CGT GC′) and SapphireAmp Fast PCR Master Mix (Takara, Shiga, Japan). he resultant sox2 PCR products were electrophoresed on agarose els, visualized with ethidium bromide, and captured using the eneGenius 2 Bio Imaging System (Syngene, MD, USA).



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    99
    Toyobo revertra ce reverse transcription enzyme
    Revertra Ce Reverse Transcription Enzyme, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/revertra+ce+reverse+transcription+enzyme/ReverTra+Ace+qPCR+RT+Kit/pm25150399-42-61-66
    Average 99 stars, based on 1 article reviews
    revertra ce reverse transcription enzyme - by Bioz Stars, 2026-09
    99/100 stars
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